Optimization of Probiotic Lactobacilli Production for In-Feed Supplementation to Feedlot Cattle

The selection of probiotic bacteria based on their beneficial characteristics does not necessarily mean they can be later scaled up and used for technological applications formula design. Three strains—Lactobacillus acidophilus CRL2074, Limosilactobacillus fermentum CRL2085, Limosolactobacillus mucosae CRL2069, originally isolated from feedlot cattle feces—have demonstrated when as in-feed probiotics. Therefore, the current study was conducted to develop a low-cost culture medium optimize growth conditions enhance biomass production. also sought identify appropriate cryoprotective agents sustain high functional cell numbers after freeze drying. A central composite design applied determine optimal composition. This yielded simplified, effective containing 3% molasses industrial yeast extracts (0.5 2.5%) carbon nitrogen sources, which were added basal each strain. Established production at 37 °C, without agitation, pH-controlled CRL2085 CRL2069 strains, free pH CRL2074 strain, allowed us obtain yields 12.95, 18.20, 12.25 g, respectively, 24-h incubation, compared with MRS medium. In addition, effect selected strain-dependent. Thus, highest viability (109–1010 CFU/g), stability during 30-d storage, survival rate (88–99%) achieved 10% MSG (monosodium glutamate), sucrose + fructose trehalose WPC (whey protein concentrate) MSG, 1.2% trehalose, drying respectively. Moreover, strains retained functionality hydrophobic evaluated. These results highlight need perform strain-specific evaluation critical factors involved in large-scale lactobacilli storage processes.